苹果赤霉素信号转导因子MdGAMYB的克隆和表达分析

以‘长富2号’苹果为试验材料,从其短枝顶芽中克隆得到1个赤霉素信号转导因子MdGAMYB,对其进行生物信息学和表达分析。结果表明,MdGAMYB的开放阅读框(ORF)长度为1 656bp,编码551个氨基酸,蛋白质分子量为59.741 kD。生物信息学分析表明MdGAMYB编码的蛋白存在多个糖基化位点和磷酸化位点;序列分析表明,Md GAYMB和其他物种的GAMYB蛋白有很高的相似性,均含有保守的R2R3 DNA结合域和GAMYB家族所特有的Box1,Box2和Box3保守区域;系统进化分析表明,Md GAYMB与梨、梅花、草莓、枣和葡萄等的GAMYB蛋白具有较高的同源性。实时荧光定量PCR分析表明,Md GAYMB具有组织表达特异性,在叶片、花和芽中的表达量较高。外源GA3处理抑制了花芽孕育和翌年成花,抑制MdGAMYB的表达。在易成花品种‘烟富6号’中的表达量高于难成花品种‘长富2号’。     

苹果‘长富2号’开花调控转录因子基因MdIDD7的克隆及表达分析

以‘长富2号’苹果为试验材料,采用RT-PCR的方法,从其芽中克隆得到INDETERMINATE DOMAIN(IDD)转录因子基因MdIDD7,其开放阅读框长度为1 626 bp,编码541个氨基酸。序列比对和结构域分析表明,该转录因子含有1个核定位信号和4个高度保守的锌指蛋白结构域。系统进化树分析表明,MdIDD7与白梨(Pyrus×bretschneideri,XP_009364602.1)、桃(Prunus persica,XP_007225628.1)和梅(Prunus mume,XP_008220893.1)聚在一起。实时荧光定量PCR表明,MdIDD7在‘长富2号’不同组织(茎、叶、花、果和芽)中均有表达,其中芽的表达量最高。花后40~60 d,MdIDD7在易成花品种‘烟富6号’芽中表达量显著高于难成花品种‘长富2号’。"小年"树顶芽组织中MdIDD7的表达量显著高于"大年"树。在花芽诱导前期,外源GA处理诱导MdIDD7下调表达,而蔗糖处理诱导其上调表达,说明MdIDD7响应激素和糖信号,促进苹果成花。     

玉米霜霉病的侵染途径及防治措施

系统综述了玉米霜霉病的症状表现、发生规律和防治措施等方面的研究进展.加强进境植物检疫、及时清除病残体和选育高抗的玉米品种是防治玉米霜霉病的重中之重.     

不同激素对碧云茶树丛生芽增殖培养的影响实验

为了获得碧云茶树的组培技术,加快其优良品种繁殖速度,2010～2011年进行了不同浓度的细胞分裂素6-BA和生长素NAA对碧云茶树丛生芽增殖培养的影响。结果表明,以MS为基本培养基,当6-BA为2.0 mg/L,NAA为0.2 mg/L时,对碧云茶树芽的诱导和增殖最有利,增殖率可达2.84;其中NAA差异不显著,6-BA差异显著。     

Mapping soil salinity and pH across an estuarine and alluvial plain using electromagnetic and digital elevation model data

Increasing pressures from agriculture and urbanization have resulted in drainage of many floodplains along the eastern Australian coastline, which are underlain by sulphidic sediments, to lower water tables and reduce soil salinity. This leads to oxidation of the sediments with a rapid decline in pH and an increase in salinity. Accurately mapping soil salinity and pH in coastal acid sulphate soil (CASS) landscapes is therefore important. One required map is the extent of highly acidic (i.e. pH < 4.5) areas, so that the application of alkaline amendments (e.g. lime) to neutralize the acid produced can be specifically targeted to the variation in pH. One approach is to use digital soil mapping (DSM) using ancillary information, such as an EM38, digital elevation models (DEM – elevation) and trend surface parameters (east and north). We used an EM38 in the horizontal (EM38h) and vertical (EM38v) modes together with elevation data to develop multiple linear regressions (MLR) for predicting EC_1:5 and pH. For pH, best results were achieved when the EM38 EC_a data were log‐transformed. By comparing MLR models using REML analysis, we found that using all ancillary data was optimal for mapping EC_1:5, whereas the best predictors for pH were north, log‐EM38v and elevation. Using residual maximum likelihood (REML), the final EC_1:5 and pH maps produced were consistent with previously defined soil landscape units, particularly CASS. The DSM approach used is amenable for mapping saline soils and identifying areas requiring the application of lime to manage acidic soil conditions in CASS landscape.     

Soil salinity related to physical soil characteristics and irrigation management in four Mediterranean irrigation districts

Irrigated agriculture is threatened by soil salinity in numerous arid and semiarid areas of the Mediterranean basin. The objective of this work was to quantify soil salinity through electromagnetic induction (EMI) techniques and relate it to the physical characteristics and irrigation management of four Mediterranean irrigation districts located in Morocco, Spain, Tunisia and Turkey. The volume and salinity of the main water inputs (irrigation and precipitation) and outputs (crop evapotranspiration and drainage) were measured or estimated in each district. Soil salinity (EC_e) maps were obtained through electromagnetic induction surveys (EC_a readings) and district-specific EC_a-EC_e calibrations. Gravimetric soil water content (WC) and soil saturation percentage (SP) were also measured in the soil calibration samples. The EC_a-EC_e calibration equations were highly significant (P < 0.001) in all districts. EC_a was not significantly correlated (P > 0.1) with WC, and was only significantly correlated (P < 0.1) with soil texture (estimated by SP) in Spain. Hence, EC_a mainly depended upon EC_e, so that the maps developed could be used effectively to assess soil salinity and its spatial variability. The surface-weighted average EC_e values were low to moderate, and ranked the districts in the order: Tunisia (3.4 dS m⁻¹) > Morocco (2.2 dS m⁻¹) > Spain (1.4 dS m⁻¹) > Turkey (0.45 dS m⁻¹). Soil salinity was mainly affected by irrigation water salinity and irrigation efficiency. Drainage water salinity at the exit of each district was mostly affected by soil salinity and irrigation efficiency, with values very high in Tunisia (9.0 dS m⁻¹), high in Spain (4.6 dS m⁻¹), moderate in Morocco (estimated at 2.6 dS m⁻¹), and low in Turkey (1.4 dS m⁻¹). Salt loads in drainage waters, calculated from their salinity (EC_dw) and volume (Q), were highest in Tunisia (very high Q and very high EC_dw), intermediate in Turkey (extremely high Q and low EC_dw) and lowest in Spain (very low Q and high EC_dw) (there were no Q data for Morocco). Reduction of these high drainage volumes through sound irrigation management would be the most efficient way to control the off-site salt-pollution caused by these Mediterranean irrigation districts.     

鼠笼异步磁力联轴器在耐高温磁力泵中的应用

为了对耐高温磁力泵进行设计和研究,保证磁力泵能够顺利启动和正常工作,对磁力泵中鼠笼转子异步磁力联轴器和泵的机械性能进行了对比分析.根据泵的基本性能公式,推导了泵的转矩与转速之间的关系.利用Ansoft Maxwell软件对鼠笼转子异步磁力联轴器进行有限元瞬态分析,得到转矩和转差率之间的关系,并分别对磁力联轴器的启动和正常工作两个阶段的磁场、扭矩进行了研究.通过将泵与磁力联轴器的性能进行综合分析,得出不同的磁力泵在不同转速下的扭矩、转差率等参数,并得出特定参数条件下泵与磁力联轴器的最优组合.试制出耐高温磁力泵样机进行试验验证,试验结果表明：在泵达到最高效率时,磁力泵的试验扭矩与理论分析得到的扭矩误差较小,说明磁力泵达到了最优化的设计结果,并验证了理论分析的正确性.     

基于电磁感应的典型干旱区土壤盐分时空变异快速诊断

选取南疆典型干旱区研究其土壤盐分时空变异,在研究区域选取校正点测定表观电导率(ECa),并同时采集土壤样品,研究表明土壤盐分含量与大地表观电导率具有良好相关性(R =0.935),可由此建立土壤盐分电磁感应解译模型.构建移动式磁感调查系统对研究区域进行调查,获取足量表观电导率信息,结合GIS和地统计等相关知识研究土壤盐...     

攀枝花市麻疯树老树特征的初步研究

本文初步研究了攀枝花市不同年龄与生境的麻疯树老树,分析了老树特征与衰老的环境诱导结构限制现象。结果表明：（1）麻疯树衰老树体相对地径（16 cm～62 cm）、树龄（15 a～41 a）、冠幅（3 m×3 m～8.5 m×8.5m）、高度（2 m～6.96 m）较大,一级枝较大,有一定比例的枯死枝;（2）新梢小（枝径0.7 cm～1.12 cm;长度7 cm～28 cm之间）,生长量低,萌生枝稀少,生殖枝比例偏低（趋近于30%以下）;（3）单枝坐果量低（0～3个）,干果重量1.65 g,千粒重490 g,壳比例30%,适合度偏低;（4）生境肥沃时树龄偏大,衰老程度弱,而生境瘠薄时树龄小,衰老程度强烈;（5）不考虑环境异质性,则衰老为树体结构限制的结果,新梢大小小于分支阈值,丧失了树体发育的能力而成为限制生长的因素。综合分析,麻疯树老树指标是分支率,在分支率为1时衰老启动;形态上衰老为环境诱导结构限制的产物,而结构限制可能是衰老的主观因素。     

外源GA_3对白桦成花基因影响的定量PCR分析

植物开花是高等植物由营养生长向生殖生长转变的重要生理过程,是个体发育和后代繁衍的中心环节。赤霉素是调节植物生长发育的五大激素之一(Takahashi et al.,1991),赤霉素处理也是人工调控植物成花的最有效途径之一,因而成为近年来成花